Matlock have already knocked Eastwood out of the cup and stolen a point of them back in August.
Matlock took the lead after 47 seconds.
Anton Daniels was the hero on Wednesday night when he score the winner however today he was the villain when he got sent of after 19 minutes for spitting.
HT ECFC 0-1 Matlock Town Reserves
The second half was dominated by Eastwood but numerous missed chances proved costly as Matlock sealed the victory seven minutes from time.
FT ECFC 0-2 Matlock Town Reserves
1 Andy Fretwell
2 Jake Danby
3 Ryan Williams
17 Matt McCaul
5 Jordan Leeming
6 Jack Havron
7 Dave Worger
8 Daniel Guild
9 Warren Hatfield
10 Anton Daniels
11 Tim Adcock
12 James Hallam
14 Ellis Evans
15 Jak Lightly
Man Of The Match Tim Adcock